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mouse anti collagen iii  (Danaher Inc)


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    Structured Review

    Danaher Inc mouse anti collagen iii
    Mouse Anti Collagen Iii, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+collagen+iii/pm38999972-342-37-40
    Average 86 stars, based on 1 article reviews
    mouse anti collagen iii - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Biochemical and Histological Differences between Longitudinal and Vertical Fibres of Dupuytren's Palmar Aponeurosis and Innovative Clinical Implications.
    Article Snippet: The slides for Collagen I were treated using an incubation of EDTA (Ethylenediaminetetraacetate—Sigma Aldrich, St. Louis, MO, USA) pH 9, at 95 °C for 15 min, to permit the antigen retrieval, and were then washed in PBS. .. Following a 1 h incubation in blocking solution (PBS + 0.2% bovine serum albumin (BSA)), all the slides were incubated with the following primary antibodies overnight at 4 °C: Goat Anti-Collagen I (Southern-Biotech (Birmingham, AL, USA), 1:400); Mouse Anti-Collagen III (Abcam (Cambridge, UK), 1:300); Mouse Anti-Human Muscle Actin (Clone 1A4-Agilent Dako (Santa Clara, CA, USA), 1:200); Rabbit Anti-Human Von Willebrand Factor (vWF-Agilent Dako, 1:600); Monoclonal Mouse anti-D2-40 (1:100), Monoclonal Mouse Anti-human CD68 (Dako-Agilent, 1:3000). .. After repetitive PBS washing, the sections were incubated with the secondary antibodies conjugated with HRP (Horseradish Peroxidase) for 1 h in PBS + 0.2% BSA/Rabbit Anti-Goat (Jackson ImmunoResearch (West Grove, PA, USA), 1:300); Goat Anti-Mouse (Jackson ImmunoResearch, 1:500); Goat Anti-Rabbit (Jackson ImmunoResearch, 1:250).

    Article Title: Biochemical and Histological Differences between Longitudinal and Vertical Fibres of Dupuytren’s Palmar Aponeurosis and Innovative Clinical Implications
    Article Snippet: The slides for Collagen I were treated using an incubation of EDTA (Ethylenediaminetetraacetate—Sigma Aldrich, St. Louis, MO, USA) pH 9, at 95 °C for 15 min, to permit the antigen retrieval, and were then washed in PBS. .. Following a 1 h incubation in blocking solution (PBS + 0.2% bovine serum albumin (BSA)), all the slides were incubated with the following primary antibodies overnight at 4 °C: Goat Anti-Collagen I (Southern-Biotech (Birmingham, AL, USA), 1:400); Mouse Anti-Collagen III (Abcam (Cambridge, UK), 1:300); Mouse Anti-Human Muscle Actin (Clone 1A4-Agilent Dako (Santa Clara, CA, USA), 1:200); Rabbit Anti-Human Von Willebrand Factor (vWF-Agilent Dako, 1:600); Monoclonal Mouse anti-D2-40 (1:100), Monoclonal Mouse Anti-human CD68 (Dako-Agilent, 1:3000). .. After repetitive PBS washing, the sections were incubated with the secondary antibodies conjugated with HRP (Horseradish Peroxidase) for 1 h in PBS + 0.2% BSA/Rabbit Anti-Goat (Jackson ImmunoResearch (West Grove, PA, USA), 1:300); Goat Anti-Mouse (Jackson ImmunoResearch, 1:500); Goat Anti-Rabbit (Jackson ImmunoResearch, 1:250).

    Article Title: Biochemical and Histological Differences between Longitudinal and Vertical Fibres of Dupuytren's Palmar Aponeurosis and Innovative Clinical Implications.
    Article Snippet: The slides for Collagen I were treated using an incubation of EDTA (Ethylenediaminetetraacetate—Sigma Aldrich, St. Louis, MO, USA) pH 9, at 95 ◦C for 15 min, to permit the antigen retrieval, and were then washed in PBS. .. Following a 1 h incubation in blocking solution (PBS + 0.2% bovine serum albumin (BSA)), all the slides were incubated with the following primary antibodies overnight at 4 ◦C: Goat Anti-Collagen I (Southern-Biotech (Birmingham, AL, USA), 1:400); Mouse Anti-Collagen III (Abcam (Cambridge, UK), 1:300); Mouse Anti-Human Muscle Actin (Clone 1A4-Agilent Dako (Santa Clara, CA, USA), 1:200); Rabbit Anti-Human Von Willebrand Factor (vWF-Agilent Dako, 1:600); Monoclonal Mouse anti-D2-40 (1:100), Monoclonal Mouse Anti-human CD68 (Dako-Agilent, 1:3000). .. After repetitive PBS washing, the sections were incubated with the secondary antibodies conjugated with HRP (Horseradish Peroxidase) for 1 h in PBS + 0.2% BSA/Rabbit AntiGoat (Jackson ImmunoResearch (West Grove, PA, USA), 1:300); Goat Anti-Mouse (Jackson ImmunoResearch, 1:500); Goat Anti-Rabbit (Jackson ImmunoResearch, 1:250).

    Blocking Assay:

    Article Title: Biochemical and Histological Differences between Longitudinal and Vertical Fibres of Dupuytren's Palmar Aponeurosis and Innovative Clinical Implications.
    Article Snippet: The slides for Collagen I were treated using an incubation of EDTA (Ethylenediaminetetraacetate—Sigma Aldrich, St. Louis, MO, USA) pH 9, at 95 °C for 15 min, to permit the antigen retrieval, and were then washed in PBS. .. Following a 1 h incubation in blocking solution (PBS + 0.2% bovine serum albumin (BSA)), all the slides were incubated with the following primary antibodies overnight at 4 °C: Goat Anti-Collagen I (Southern-Biotech (Birmingham, AL, USA), 1:400); Mouse Anti-Collagen III (Abcam (Cambridge, UK), 1:300); Mouse Anti-Human Muscle Actin (Clone 1A4-Agilent Dako (Santa Clara, CA, USA), 1:200); Rabbit Anti-Human Von Willebrand Factor (vWF-Agilent Dako, 1:600); Monoclonal Mouse anti-D2-40 (1:100), Monoclonal Mouse Anti-human CD68 (Dako-Agilent, 1:3000). .. After repetitive PBS washing, the sections were incubated with the secondary antibodies conjugated with HRP (Horseradish Peroxidase) for 1 h in PBS + 0.2% BSA/Rabbit Anti-Goat (Jackson ImmunoResearch (West Grove, PA, USA), 1:300); Goat Anti-Mouse (Jackson ImmunoResearch, 1:500); Goat Anti-Rabbit (Jackson ImmunoResearch, 1:250).

    Article Title: Biochemical and Histological Differences between Longitudinal and Vertical Fibres of Dupuytren’s Palmar Aponeurosis and Innovative Clinical Implications
    Article Snippet: The slides for Collagen I were treated using an incubation of EDTA (Ethylenediaminetetraacetate—Sigma Aldrich, St. Louis, MO, USA) pH 9, at 95 °C for 15 min, to permit the antigen retrieval, and were then washed in PBS. .. Following a 1 h incubation in blocking solution (PBS + 0.2% bovine serum albumin (BSA)), all the slides were incubated with the following primary antibodies overnight at 4 °C: Goat Anti-Collagen I (Southern-Biotech (Birmingham, AL, USA), 1:400); Mouse Anti-Collagen III (Abcam (Cambridge, UK), 1:300); Mouse Anti-Human Muscle Actin (Clone 1A4-Agilent Dako (Santa Clara, CA, USA), 1:200); Rabbit Anti-Human Von Willebrand Factor (vWF-Agilent Dako, 1:600); Monoclonal Mouse anti-D2-40 (1:100), Monoclonal Mouse Anti-human CD68 (Dako-Agilent, 1:3000). .. After repetitive PBS washing, the sections were incubated with the secondary antibodies conjugated with HRP (Horseradish Peroxidase) for 1 h in PBS + 0.2% BSA/Rabbit Anti-Goat (Jackson ImmunoResearch (West Grove, PA, USA), 1:300); Goat Anti-Mouse (Jackson ImmunoResearch, 1:500); Goat Anti-Rabbit (Jackson ImmunoResearch, 1:250).

    Article Title: Biochemical and Histological Differences between Longitudinal and Vertical Fibres of Dupuytren's Palmar Aponeurosis and Innovative Clinical Implications.
    Article Snippet: The slides for Collagen I were treated using an incubation of EDTA (Ethylenediaminetetraacetate—Sigma Aldrich, St. Louis, MO, USA) pH 9, at 95 ◦C for 15 min, to permit the antigen retrieval, and were then washed in PBS. .. Following a 1 h incubation in blocking solution (PBS + 0.2% bovine serum albumin (BSA)), all the slides were incubated with the following primary antibodies overnight at 4 ◦C: Goat Anti-Collagen I (Southern-Biotech (Birmingham, AL, USA), 1:400); Mouse Anti-Collagen III (Abcam (Cambridge, UK), 1:300); Mouse Anti-Human Muscle Actin (Clone 1A4-Agilent Dako (Santa Clara, CA, USA), 1:200); Rabbit Anti-Human Von Willebrand Factor (vWF-Agilent Dako, 1:600); Monoclonal Mouse anti-D2-40 (1:100), Monoclonal Mouse Anti-human CD68 (Dako-Agilent, 1:3000). .. After repetitive PBS washing, the sections were incubated with the secondary antibodies conjugated with HRP (Horseradish Peroxidase) for 1 h in PBS + 0.2% BSA/Rabbit AntiGoat (Jackson ImmunoResearch (West Grove, PA, USA), 1:300); Goat Anti-Mouse (Jackson ImmunoResearch, 1:500); Goat Anti-Rabbit (Jackson ImmunoResearch, 1:250).



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    Effects of ethyl pyruvate on the expression of fibrotic and cytostatic markers in HDFs and KFs. ( A ) Treatment of TGF-β significantly increased the expression <t>of</t> <t>collagen</t> <t>I/III</t> mRNA. Treating ethyl pyruvate significantly decreased mRNA expression as the dose increased. ( B ) Similarly, treating ethyl pyruvate to KFs showed reduced collagen I/III mRNA expression (* p < 0.05).
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    Image Search Results


    Effects of ethyl pyruvate on the expression of fibrotic and cytostatic markers in HDFs and KFs. ( A ) Treatment of TGF-β significantly increased the expression of collagen I/III mRNA. Treating ethyl pyruvate significantly decreased mRNA expression as the dose increased. ( B ) Similarly, treating ethyl pyruvate to KFs showed reduced collagen I/III mRNA expression (* p < 0.05).

    Journal: International Journal of Molecular Sciences

    Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids

    doi: 10.3390/ijms25115844

    Figure Lengend Snippet: Effects of ethyl pyruvate on the expression of fibrotic and cytostatic markers in HDFs and KFs. ( A ) Treatment of TGF-β significantly increased the expression of collagen I/III mRNA. Treating ethyl pyruvate significantly decreased mRNA expression as the dose increased. ( B ) Similarly, treating ethyl pyruvate to KFs showed reduced collagen I/III mRNA expression (* p < 0.05).

    Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), mouse anti-collagen type III (Sigma-Aldrich), mouse anti-elastin (Sigma-Aldrich), and mouse anti-fibronectin (Santa Cruz Biotechnology).

    Techniques: Expressing

    Effect of ethyl pyruvate on expression of fibrosis-related protein. ( A ) Western blot analysis of collagen I, III, TGF-β, and p-SMAD2/3 expression was performed; ( B ) upon densitometric analysis, collagen I, III, TGF-β, and p-SMAD2/3 protein expression levels decreased when KF cells were treated with 20mM of ethyl pyruvate (* p < 0.05).

    Journal: International Journal of Molecular Sciences

    Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids

    doi: 10.3390/ijms25115844

    Figure Lengend Snippet: Effect of ethyl pyruvate on expression of fibrosis-related protein. ( A ) Western blot analysis of collagen I, III, TGF-β, and p-SMAD2/3 expression was performed; ( B ) upon densitometric analysis, collagen I, III, TGF-β, and p-SMAD2/3 protein expression levels decreased when KF cells were treated with 20mM of ethyl pyruvate (* p < 0.05).

    Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), mouse anti-collagen type III (Sigma-Aldrich), mouse anti-elastin (Sigma-Aldrich), and mouse anti-fibronectin (Santa Cruz Biotechnology).

    Techniques: Expressing, Western Blot

    Immunohistochemical analysis of collagen I and III, fibronectin, and elastin in ethyl pyruvate-treated keloid spheroids ( n = 3). ( A ) Representative images of collagen I and III, fibronectin, and elastin IHC staining of keloid spheroids treated with ethyl pyruvate (10, 20, and 40 mM). ( B ) Semi-quantification of protein expression is shown. Collagen I and III, fibronectin, and elastin were significantly decreased in keloid spheroids following ethyl pyruvate application. Asterisks (*) over each column signify a statistically significant difference compared to the control (0 mM, white bar) group (* p < 0.05). ( C ) Reduction in collagen I protein expression can be seen with western blot analysis.

    Journal: International Journal of Molecular Sciences

    Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids

    doi: 10.3390/ijms25115844

    Figure Lengend Snippet: Immunohistochemical analysis of collagen I and III, fibronectin, and elastin in ethyl pyruvate-treated keloid spheroids ( n = 3). ( A ) Representative images of collagen I and III, fibronectin, and elastin IHC staining of keloid spheroids treated with ethyl pyruvate (10, 20, and 40 mM). ( B ) Semi-quantification of protein expression is shown. Collagen I and III, fibronectin, and elastin were significantly decreased in keloid spheroids following ethyl pyruvate application. Asterisks (*) over each column signify a statistically significant difference compared to the control (0 mM, white bar) group (* p < 0.05). ( C ) Reduction in collagen I protein expression can be seen with western blot analysis.

    Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), mouse anti-collagen type III (Sigma-Aldrich), mouse anti-elastin (Sigma-Aldrich), and mouse anti-fibronectin (Santa Cruz Biotechnology).

    Techniques: Immunohistochemical staining, Immunohistochemistry, Expressing, Western Blot